منابع مشابه
Serial Femtosecond Crystallography
X-ray free-electron lasers produce brief flashes of X-rays that are of about a billion times higher peak brightness than achievable from storage ring sources. Such a tremendous jump in X-ray source capabilities, that came in 2009 when the Linac Coherent Light Source begun operations, was unprecedented in the history of X-ray science. Protein structure determination through the method of macromo...
متن کاملExperimental phasing of serial femtosecond crystallography data
A synopsis of and prospects for de novo phasing using diffraction data collected at X-ray free-electron lasers are given.
متن کاملSerial femtosecond crystallography: the first five years
Protein crystallography using synchrotron radiation sources has had a tremendous impact on biology, having yielded the structures of thousands of proteins and given detailed insight into their mechanisms. However, the technique is limited by the requirement for macroscopic crystals, which can be difficult to obtain, as well as by the often severe radiation damage caused in diffraction experimen...
متن کاملAsymmetry in serial femtosecond crystallography data
Serial crystallography is an increasingly important approach to protein crystallography that exploits both X-ray free-electron laser (XFEL) and synchrotron radiation. Serial crystallography recovers complete X-ray diffraction data by processing and merging diffraction images from thousands of randomly oriented non-uniform microcrystals, of which all observations are partial Bragg reflections. R...
متن کاملSerial femtosecond crystallography datasets from G protein-coupled receptors
We describe the deposition of four datasets consisting of X-ray diffraction images acquired using serial femtosecond crystallography experiments on microcrystals of human G protein-coupled receptors, grown and delivered in lipidic cubic phase, at the Linac Coherent Light Source. The receptors are: the human serotonin receptor 2B in complex with an agonist ergotamine, the human δ-opioid receptor...
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ژورنال
عنوان ژورنال: Nature Reviews Methods Primers
سال: 2022
ISSN: ['2662-8449']
DOI: https://doi.org/10.1038/s43586-022-00153-3